by Francisco Andrade » September 13th, 2003, 1:44 pm
Hi Louis,
Thanks for the help, I´ll try to boost the B vitamins. I do have the recipe, look :
1000 ml Sea Water ( as clean as possible), add:
- NaNO3 75mg
- Na2HPO4 . 7H2O 5mg
- Thiamin 100 ug
- Biotin 0,5 ug
- B12 vitamin 0,5 ug
- Trace metals solution 1ml
Trace metals solution
1000ml distilled water, add:
- Na2 EDTA 8,72 g
- FeCl3 . 6H2O 6,3g
- MnCl2 . 4H2O 360mg
- CuSO4 . 5H2O 20mg
- ZnSO4 . 7H2O 44mg
- CoCl2 . 6H2O 20mg
- NaMoO4 . 2H2O 12 mg
With this medium the algae cultures did great. But to buy all the salts is expensive, especially when I have to buy 100g and need just 20mg to make 1000 l of culture medium.
See ya